Probing Protein Conformation for Antibiotic Development

We have developed an electrochemical sandwich assay to monitor conformational changes upon small molecule binding to a protein. This platform relies on a recognition element that binds to a specific conformation of the protein of interest. This platform has especially high sensitivity due to inherent signal amplification from E. coli engineered to surface express the protein of interest. This platform is being expanded to evaluate small molecule binding to proteins involved in antibiotic resistance, along with other ‘undruggable’ targets.